Specimen Information

Autoclave Monitoring

Purpose: The ATTEST
is used as a biological indicator for saturated steam sterilization cycles. The spore phase of the chosen microorganism is resistant to moist heat sterilization and is used  to validate the lethality of steam sterilization. You much have a UVM Medical Center Account Number to order this test. Call Laboratory Customer Service if you have questions.

Precautions:  ATEST Ampules contain live cultures and should be handled with care toprevent breakage. Use ampule only once.

Storage: Store at ambient temperature, between 59 – 86 f○. Do not store near sterilants or other chemicals. ATTESTs are good for two years after manufacture date,  which is printed on the box.

Procedure:

1. Place unopened ATEST in the autoclave. Vial may be included with a normal load or they may be tested alone.
2. Follow usual procedures for running a load for sterilization.
3. Remove vial when cool.
4. Check the chemical indicator on the label for a color change from rose to brown.
5. Each time an ATEST is submitted, a control must be included. Use one ATEST that has NOT been autoclaved and with a sharpie mark “Control” on it.
6. Complete laboratory requisition. In the name field on the requisition put “SPORE TESTING”.  Each specimen should also be uniquely identified for your  office’s record keeping. Check off your 96 account number.
7. Take ATEST and wrap them in a paper towel. Place in specimen bag. Place bag in REFRIGERATOR. Send with next shipment of specimens. Specimens must remain refrigerated in transport.
8. Test vials must be sent to lab within 24 hours of sterilization.
9. Results will be returned within two weeks.

Interpretation: Spore growth indicates autoclave is not functioning properly. Consult manufacturer before using autoclave.