Sign in →

BCR/ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies

Additional Codes

Primary ID

Epic Code

Mayo Test ID

P190

LAB3690 BA190

 

Reporting Name

BCR/ABL1, p190, Quant, Monitor

Useful For

Monitoring response to therapy in patients with known e1/a2 BCR/ABL1 (p190) fusion forms

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Advisory Information


This test should not be used to screen for BCR/ABL1 fusions at the time of diagnosis; order either BADX / BCR/ABL1, Qualitative, Diagnostic Assay, Varies; or BCRFX / BCR/ABL1 Qualitative Diagnostic Assay with Reflex to BCR/ABL1 p190 Quantitative Assay or BCR/ABL1 p210 Quantitative Assay, Varies should be ordered for that purpose.

 

To monitor patients carrying BCR/ABL1 fusion forms coding for the p210 protein, which includes most patients with chronic myeloid leukemia (CML); order BCRAB / BCR/ABL, p210, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Chronic Myelogenous Leukemia (CML), Varies.



Shipping Instructions


Refrigerate specimens must arrive within 5 days (120 hours) of collection, and ambient specimens must arrive within 3 days (72 hours) of collection. Collect and package specimen as close to shipping time as possible.



Necessary Information


The following information is required:

1. Pertinent clinical history including if the patient has a diagnosis of chronic myelogenous leukemia or other BCR/ABL1-positive neoplasm

2. Date of collection

3. Specimen source (blood or bone marrow)



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Whole blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 10 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.


Specimen Minimum Volume

Blood: 4 mL
Bone marrow: 2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 5 days PURPLE OR PINK TOP/EDTA
  Ambient  72 hours PURPLE OR PINK TOP/EDTA

Reference Values

The presence or absence of the BCR/ABL1 mRNA fusion form producing the p190 fusion protein is reported. If positive, the level is reported as the ratio of BCR/ABL1 (p190) transcript to ABL1 transcript in the form of a percentage.

Day(s) and Time(s) Performed

Monday through Saturday; a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81207

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BA190 BCR/ABL1, p190, Quant, Monitor In Process

 

Result ID Test Result Name Result LOINC Value
39470 BCR/ABL1 p190 Result No LOINC Needed
MP002 Specimen Type 31208-2
19765 Interpretation 69047-9

Analytic Time

4 days

Specimen Retention Time

RNA 3 months

Reject Due To

Gross hemolysis Reject
Moderately to severely clotted Reject

NY State Approved

Yes

Method Name

Quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Forms

1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Testing Algorithm

See BCR/ABL1 Ordering Guide for Blood and Bone Marrow in Special Instructions.