Sign in →

Estradiol, Serum

Important Note

For pediatric patients only.

Additional Codes

Primary ID

Epic Code

Mayo Test ID

NESTRA

LAB2482

EEST

 

Reporting Name

Estradiol, Mass Spectrometry, S

Useful For

All applications that require moderately sensitive measurement of estradiol:

-Evaluation of hypogonadism and oligo-amenorrhea in females

-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)

-In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women

-Evaluation of feminization, including gynecomastia, in males

-Diagnosis of estrogen-producing neoplasms in males and, to a lesser degree, females

-As part of the diagnosis and workup of precocious and delayed puberty in females, and, to a lesser degree, males

-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism (eg, aromatase deficiency and 17 alpha-hydroxylase deficiency)

-As an adjunct to clinical assessment, imaging studies and bone mineral density measurement in the fracture risk assessment of postmenopausal women, and, to a lesser degree, older men

-Monitoring low-dose female hormone replacement therapy in postmenopausal women

-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)

Testing Algorithm

See Steroid Pathways in Special Instructions.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Specimen Required


Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.2 mL

Collection Instructions:

1. Centrifuge and remove serum from red blood cells within 2 hours of draw.

2. Aliquot serum to submission container.

Additional Information: See Steroid Pathways in Special Instructions.


Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Special Instructions

Reference Values

CHILDREN*

1-14 days: Estradiol levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.

Males

Tanner Stages#

Mean Age

Reference Range

Stage I (>14 days and prepubertal)

7.1 years

Undetectable-13 pg/mL

Stage II

12.1 years

Undetectable-16 pg/mL

Stage III

13.6 years

Undetectable-26 pg/mL

Stage IV

15.1 years

Undetectable-38 pg/mL

Stage V

18 years

10-40 pg/mL

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (± 2) years. For boys, there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

 

Females

Tanner Stages#

Mean Age

Reference Range

Stage I (>14 days and prepubertal)

7.1 years

Undetectable-20 pg/mL

Stage II

10.5 years

Undetectable-24 pg/mL

Stage III

11.6 years

Undetectable-60 pg/mL

Stage IV

12.3 years

15-85 pg/mL

Stage V

14.5 years

15-350 pg/mL**

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (± 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

 

*The reference ranges for children are based on the published literature(1,2), cross-correlation of our assay with assays used to generate the literature data, and on our data for young adults.

 

ADULTS

Males: 10-40 pg/mL

Females

Premenopausal: 15-350 pg/mL**

Postmenopausal: <10 pg/mL

**E2 levels vary widely through the menstrual cycle.

Conversion factor

E2: pg/mL x 3.676=pmol/L (molecular weight=272)

 

For SI unit Reference Values, see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) and Time(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

82670

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EEST Estradiol, Mass Spectrometry, S 2243-4

 

Result ID Test Result Name Result LOINC Value
81816 Estradiol, Mass Spectrometry, S 2243-4

Analytic Time

2 days

Specimen Retention Time

2 weeks

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK
Other Serum gel or SST tube

NY State Approved

Yes

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)