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Test Code GPST GENE PANEL SOLID TUMOR TESTING

Important Note

The Request for Genomic Analysis order will result in assessment of sample adequacy for genomic analysis, the result of which will post to the patient record. Should a sample be deemed adequate for genomic analysis, the genomic medicine laboratory will reflexively initiate the appropriate solid tumor testing.
 
The Genomic Medicine Laboratory at the University of Vermont Medical Center offers target capture-based next generation sequencing of 30 genes that have clinical utility across solid tumors. Pathologist assessment of sample adequacy and tumor type inform analysis via Gene Panel Solid Tumor or Gene Panel NSCLC. Non-small cell lung cancer is submitted for DNA-based and RNA-based sequencing, the latter for targeted gene fusion analysis in ALK, RET and ROS1.

The test is validated for formalin-fixed paraffin embedded tissue and all non-cell block cytology preparations.  Extracted eluates may be accepted with Pathologist approval.

Additional Codes

Primary ID Epic Code

Mayo Access ID

GPST LAB9915 N/A

 

Specimen Information

Container

Specimen

Temperature

Collect Vol

Submit Vol

Minimum Vol

Stability

Formalin fixed paraffin embedded tissue or cell block

Tissue

Ambient

 

 

 

 
Cytology Slide Preparation Slide          

All specimens must contain ≥ 10% tumor cells.

Nucleic Acid Input Amounts
DNA, 100 ng
RNA, 150 ng

 

Test Schedule / Analytical Time / Test Priority

Monday – Friday / 10 - 14 days / Not available STAT

Method

Total nucleic acid, DNA plus RNA, extracted from cytology slide preparations and formalin-fixed paraffin embedded tissue are analyzed for Gene Panel Solid Tumor and Gene Panel Solid Tumor Non-Small Cell Lung Cancer. Our DNA test employs Agilent Sure Select library preparation and target capture and the RNA sequencing portion of Gene Panel Solid Tumor Non-Small Cell Lung Cancer utilizes ArcherDx FusionPlex amplicon library preparation. Sequencing is performed on Illumina MiSeq, MiSeqDx and NextSeq instrumentation.

CPT(s)

Description CPT
Solid Tumor Gene Panel 81445

 

Instrumentation

Manual Extraction and Illumina miseq for Sequencing

Section

Genomic Medicine

Performing Location

University of Vermont Medical Center

LOINC Code Information

N/A

Gene List

Oncogenes AKT1
  ALK
  BRAF
  CTNNB1
  DDR2
  EGFR
  ERBB2
  ESR1
  FGFR1
  FGFR2
  GNA11
  GNAQ
  HRAS
  KIT
  KRAS
  MAP2K1
  MAP2K2
  MAPK1
  MET
  MTOR
  NRAS
  PDGFRA
  PIK3CA
  RET
  ROS1
Tumor Suppressor Genes CDKN2A
  PTEN
  STK11
Metabolic Genes IDH1
  IDH2

 

Is the UVMMC lab NY State Certified to perform this testing? Yes/No

No