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Test Code IMM IMMUNODEFICIENCY PANEL (T, B & NK CELL SUBSETS)

Important Note

Hemagram and differential (CBCDF) are required for absolute counts. Dual platform analysis is performed when done at the University of Vermont Laboratory. Outside clients may submit a hemagram and differential from their own instrumentation with the sample or place an order for a CBCDF and submit an EDTA for testing.  If the CBCDF will not be tested within 12 hours, also submit a properly labelled smear. 

A CBCDF must be performed within 24 hours of the subset analysis, however a CBCDF drawn at the same time is optimal.

Additional Codes

Primary ID

Epic Code

Mayo Access ID

IMM

LAB343 FAH5221

 

Specimen Information

Container

Specimen

Temperature

Collect Vol

Submit Vol

Minimum Vol

Sodium Heparin Tube Whole Blood Ambient 4 mL 2 mL 2 mL
Purple Tube (EDTA) Whole Blood Ambient 4 mL 2 mL 2 mL

Samples collected in sodium Heparin must be tested within 48 hours of collection. Samples collected in EDTA must be tested within 30 hours of collection.

Test Schedule / Analytical Time / Test Priority

Monday – Saturday / 3 days / Not available STAT

Method

Flow Cytometry

CPT(s)

Description CPT Code
CD 19 86355
CD 3                                                86359
CD 4 & CD 8 86360
CD NK 86357

 

Instrumentation

Beckman Coulter Navios

Reference Range

Pediatric Reference Ranges

 

Age

CD3%

CD4%

CD8%

CD19%

CD16+56%

Absolute CD4

0-2 Mos.

53-84%

35-64%

12-28%

6-32%

4-18%

1600-4000 cells/uL

3-5 Mos

51-77%

35-56%

12-23%

11-41%

3-14%

1800-4000 cells/uL

6-11 Mos.

49-76%

31-56%

12-24%

14-37%

3-15%

1400-4300 cells/uL

12-23 Mos.

53-75%

32-51%

14-30%

16-35%

3-15%

1300-3400 cells/uL

2-5 Yrs.

56-75%

28-47%

16-30%

14-33%

4-17%

700-2200 cells/uL

6-11 Yrs.

60-76%

31-47%

18-35%

13-17%

3-22%

650-1500 cells/uL

12-17 Yrs.

56-84%

31-52%

18-35%

6-23%

3-22%

530-1300 cells/uL

 

Adult Reference Ranges

 

Subset

%

Absolute

CD3%

62-87%

548-2118 cells/uL

CD4%

35-63%

329-1427 cells/uL

CD8%

10-35%

66-750 cells/uL

CD19%

5-22%

<488 cells/uL

CD16+56%

5-23%

72-425 cells/uL

 

Section

Immunology

Performing Location

University of Vermont Medical Center

Is the UVMMC lab NY State Certified to perform this testing?  Yes/No

Yes

LOINC Code Information

Result Code Reporting Name LOINC Code
CD3 CD3 20599-7
CD4 CD4 32516-7
CD8 CD8 32518-3
CD19 CD19 20593-0
CDNK CD16+CD56 21166-4
TOTCD3 Absolute CD3 20598-9
TOTCD4 Absolute CD4 32515-9
TOTCD8 Absolute CD8 in process
TOT19 Absolute CD19 in process
TOTNK Absolute CD16+CD56 in process