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Test Code LAB9913 FISH TESTING (FLUORESCENCE IN SITU HYBRIDIZATION)

Important Note

Commercial payors may require preauthorization for this test.

Outside clients please use Hem path/Flow Cytometry/Genetic Laboratory Form.

See Test Note below for available probes.

Submission of an order for Cytogenetic testing for congenital disorders constitutes that the ordering physician has, obtained an informed consent of the patient as required by any applicable state or federal laws with respect to the test ordered, and obtained from the patient authorization permitting UVM Medical Center to report results of each test ordered directly to the ordering physician.

Additional Codes

Epic Code Atlas Code Mayo Access ID Order LOINC
LAB9913 N/A N/A N/A

 

Specimen Information

Container Specimen Temp Collect Vol Submit Vol Min Vol
Bone Marrow Transport Media (sterile) Bone Marrow Ambient 3 mL  3 mL 0.5 mL
Sodium Heparin Tube Bone Marrow Ambient 3 mL 3 mL 0.5 mL
Sodium Heparin Tube, Adult* Whole Blood Ambient 6 mL 6 mL 3 mL
Sodium Heparin Tube, Pediatric* Whole Blood Ambient 3 mL 3 mL 1 mL
Hanks Solution Lymph Tissue/Node Ambient 1 cm3 1 cm3 0.5 cm3

*Samples should be kept at ambient temperature after collection and during transport. Containers are available from Laboratory Customer Service 847-5121.

Some FISH testing can be done on unstained fresh tissue slides (air-dried FNA smear or touch imprints). Call the Cytogenetics Laboratory at  847-3565.

Containers are available from Laboratory Customer service 847-5121, bone marrow transport media (RPMI) supply #032047, sodium heparin supply #031977, hanks solution supply #032048. Use Hematopoietic Neoplasm Lab Form, supply #032014.

Test Schedule / Analytical Time / Test Priority

Monday – Saturday / 7 days / Not available STAT

Method

Culture, Microscopy,Probe Hybridzation, and Interpretation

CPT Code(s)

Description CPT Code
Blood /Bone Marrow Culture, Neoplastic 88237
FISH DNA Probe 88271
FISH Intrp & Report Part B 88291
In Situ Hybridization, Interphase, BM or Blood: 100-300 cells 88275
In Situ Hybridization, Interphase, BM or Blood: 25-99 cells 88274
Blood Culture 88230
In Situ Hybridization, Metaphase:  10-30 cells 88273
In Situ Hybridization, Metaphase:  3-5 cells 88272

 

Instrumentation

Manual Method

Section

Cytogenetics

Performing Location

University of Vermont Medical Center

Is the UVMMC lab NY State Certified to perform this testing?  Yes/No

Yes

Test Note

FISH Testing (Fluorescence In-Situ Hybridization):

  • Fluorescent probes can be used to characterize chromosome abnormalities associated with specific hematologic diseases.
  • Reporting time is 7 days.
  • FISH testing can be done on the same sample submitted for chromosome evaluation or it can be ordered alone.

Some FISH testing can be done on unstained fresh tissue slides (air-dried FNA smear or touch imprints). Call the Cytogenetics Laboratory at  847-3565.


Hematologic (Neoplasia) Oncology probes available at UVMMC:
t(8;14) MYC/IGH, Burkitt’s Lymphoma
t(8;21) RUNX1/RUNX1T1, Acute Myeloid Leukemia (AML)
t(9;22) BCR/ABL, Chronic Myelogenous Leukemia (CML)
t(11;14) CCND1/IGH, Mantle Cell Lymphoma
t(12;21) ETV6/RUNX1, Acute Lymphoblastic Leukemia (ALL)
t(14;18) BCL2/IGH, Follicular Lymphoma
t(15;17) PML/RARA, Acute Promyelocytic Leukemia (APL)
CBFB Rearrangement inv (16), AML with eosinophils
MLL Rearrangement 11q23 AML, ALL or MDS
MYC Rearrangement 8q24, Burkitt’s Lymphoma
BCL2 Rearrangement 18q21 Large B Cell Lymphoma
BCL6 Rearrangement 3q27 Large B Cell Lymphoma
CLL FISH Panel, Chronic Lymphocytic Leukemia


Congenital Syndrome probes available at UVMMC:
DiGeorge Syndrome 22q11.2 deletion or duplication
Prader-Willi/ Angel man Syndrome 15q11.2 deletion or duplication
Smith-Magenis Syndrome 17p11.2 deletion or duplication
Williams Syndrome 7q11.23 deletion or duplication
CepX/SRY Xp11.1-q11.1 Yp11.3 enumeration or rearrangement of sex chromosomes


Send out FISH tests: require a separate sodium heparin tube (1-2mL)
Plasma Cell Proliferative Disorder, Myeloma FISH Panel