Sign in →

Microsporidia species, Molecular Detection, PCR, Varies

Important Note

Microsporidia are obligate intracellular spore forming protozoa belonging to the phylum Microspora. The genera within this phylum which have been recognized in humans include Encephalitozoan, Nosema, Pleistophora, Enterocytozoan, and Septata. Enterocytozoan bieneusi and Septata intestinalis are known to cause chronic diarrhea in HIV positive patients or other immunocompromised individuals.

Additional Codes

Orderable Codes:

Epic Code Atlas Code Mayo Test ID Order LOINC
LAB3576 MSPOR LCMSP 94332-4

Result Component(s):

Reporting Name Epic Code Atlas Code Mayo Test ID Result LOINC
Specimen Source 123010065538 MSOU31 MSPS 31208-2
Encephalitozoon species 123010065539 M36744 36744 94333-2
Enterocytozoon bieneusi 123010065540 M36745 36745 94334-0

 

Useful For

Detecting Enterocytozoon bieneusi and Encephalitozoon species in fecal and urine specimens to support the clinical diagnosis of microsporidiosis

Reporting Name

Microsporidia PCR

Specimen Type

Varies
University of Vermont Medical Center Laboratory Test Catalog Note:

Stool samples submitted for this test must be collected in a sterile container with no preservative. Stool samples submitted in O&P Collection Vials will be rejected. Ecofix preservative (Mayo Medical Laboratory) is generally not available. Contact the Microbiology Laboratory (847-5121 or 1-800-991-2799) for questions.


Ordering Guidance


For specimens other than feces or urine, MTBS / Microsporidia Stain, Varies should be ordered since it will detect other microsporidia not targeted by this assay.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Unpreserved feces

Supplies:

-Stool container, Small (Random), 4 oz (T288)

-Stool Collection Kit, Random (T635)

Container/Tube: Fecal container

Specimen Volume: 5 g

 

Specimen Type: Preserved feces

Supplies:

-ECOFIX Stool Transport Vial (Kit) (T219)

-Stool Collection Kit, Random (T635)

Container/Tube: ECOFIX preservative

Specimen Volume: 5 g

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 5 mL

Collection Instructions: Mid-stream, clean-catch, suprapubic aspirates and catheterization collections are acceptable. Submit in a clean, sterile container free from preservatives. The first portion of the voided urine (first void) is also acceptable.


Specimen Minimum Volume

Feces: 1 g
Urine: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reject Due To

Urine containing preservatives
Prostate secretions and samples
Feces in preservatives other than ECOFIX		 Reject

Reference Values

Negative

Day(s) Performed

Tuesday through Friday

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LCMSP Microsporidia PCR 94332-4

 

Result ID Test Result Name Result LOINC Value
MSPS Specimen Source 31208-2
36744 Encephalitozoon species 94333-2
36745 Enterocytozoon bieneusi 94334-0

NY State Approved

Yes

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Gastroenterology and Hepatology Test Request (T728)