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Test Code SPUTBB MYCOBACTERIUM TUBERCULOSIS, PCR

Important Note

This test requires pathology approval on AFB smear negative samples.

This test is not orderable as a stand alone test. It is a reflex test for  AFB CULTURE & SMEAR.

This test will be performed on all first time AFB positive smear sputum specimens and AFB negative smear specimens, if indicated by the ordering clinical team by answering the AOE (Ask at Order Entry) question as "Yes" to rule out tuberculosis.

This test also detects resistance to Rifampin.

This test is subject to reflex testing, see Laboratory Reflex Testing Policy.  You have the option to decline reflex testing if you believe it is not medically necessary.

Additional Codes

Orderable Codes:

Epic Code Atlas Code Mayo Access ID Order LOINC Legacy Code
N/A N/A N/A in process SPUTBB

Specimen Information

Container Specimen Temperature Collect Vol Submit Vol Min Vol Stability
Sterile Container Sputum Refrigerate 5 mL 5 mL 3 mL 48 hours

Sample must be received within 48 hours of specimen collection.

Test Schedule / Analytical Time / Test Priority

Test is run when AFB Smear is positive for the first time / Result is available the same day / AFB Smear NOT available STAT

Method

PCR

Instrumentation

GenXpert

Reference Range

No Mycobacterium tuberculosis complex DNA detected by PCR.
A single negative result has a high negative predicitve value and can be used in conjunction with other clinical findings to decide whether to remove suspected tuberculosis patients from isolation.
 

Section

Microbiology-2

Performing Location

University of Vermont Medical Center

Is the UVMMC lab NY State Certified to perform this testing?  Yes/No

No

CPT Code(s)

Description CPT Code
Mycobacterium tuberculosis, amplified probe 87556