Sign in →

Test Code TOTCD3 ABSOLUTE CD3

Important Note

Add Important Note:  Hemagram and differential (CBCDF) is required for total CD3 count (absolute).  Outside clients may submit a hemagram with differential from their own intrumentation with the sample or place and order for a CBCDF and also submit a lavender top tube (EDTA) for testing.  If the CBCDF will not be tested within 12 hours, also submit a properly labelled smear.  A CBCDF must be performed within 24 hours of the total CD3, however a CBCDF drawn at the same time is optimal.

Additional Codes

Primary ID

Epic Code

Mayo Access ID

TOTCD3

LAB2326 N/A

 

Specimen Information

Container

Specimen

Temperature

Collect Vol

Submit Vol

Minimum Vol

Stability
Purple Top (EDTA) Whole Blood Ambient 4 mL 2 mL 1 mL 30 hours
Sodium Heparin Tube Whole Blood Ambient 4 mL 2 mL 1 mL 48 hours

 

Test Schedule / Analytical Time / Test Priority

Monday – Saturday / 3 days / Not available STAT

Method

Flow Cytometry

CPT(s)

Description CPT Code
 T cells; total count                      86359

 

Instrumentation

Beckman Coulter FC500 and Beckman Coulter Navios

Reference Range

In newly transplanted patients, therapeutic depletion of CD3+ cells is considered to correspond to 25 - 50 CD3+ cells/μL.  Pediatric patients are known to have higher CD3 lymphocyte levels and may require progressively higher dosages of muromonab-CD3 to achieve therapeutic depletion.

Section

Immunology

Performing Location

University of Vermont Medical Center

Is the UVMMC lab NY State Certified to perform this testing?  Yes/No

Yes