Sign in →

Influenza Virus Type A and Type B and Respiratory Syncytial Virus (RSV) RNA, Molecular Detection, PCR, Varies

Important Note

This is for Bronchoalveolar lavage (BAL) fluid and bronchial washing specimens Only.

For other specimen types, see SARS CoV2, Flu A/B, and RSV Detection by PCR (LAB17655).

Additional Codes

Orderable Codes:

Epic Code Atlas Code Mayo Test ID Order LOINC
LAB17678 LAB17678 HPFLU 92143-7

Result Component(s):

Reporting Name Epic Code Atlas Code Mayo Test ID Result LOINC
*Influenza A/B and RSV Source 12301020341 MHPFLS HPFLS 31208-2
Influenza A virus PCR 12301020342 M610412 610412 in process
Influenza B virus PCR 12301020343 M610413 610413 in process
Respiratory Syncytial virus 12301020344 M610414 610414 in process

*Acceptable options are Bronchoalveolar Lavage (BAL) Fluid or Bronchial washings.


Ordering Guidance


Due to the nonspecific clinical presentation of influenza during the early stages of the illness, testing for other respiratory infections (eg, COVID-19) may be warranted. If this test is ordered with HPCOV / Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2), Molecular Detection, Varies, then both tests will be canceled and automatically reordered by the laboratory as SCOF4 / Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2), Influenza Virus Type A and Type B RNA, and Respiratory Syncytial Virus (RSV) Molecular Detection, PCR, Varies.



Specimen Required


Preferred:

Specimen Type: Nasopharyngeal swab

Container/Tube: Sterile container with viral transport media

Specimen Volume: Entire specimen with a minimum of 1.5 mL (maximum 3 mL) of transport media

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Swab must be placed into viral transport media (eg, M4-RT, M4 or M5), saline, or phosphate buffered saline (PBS). Media should not contain guanidine thiocyanate (GTC).

 

Specimen Type: Bronchoalveolar lavage fluid

Container/Tube: Sterile container

Specimen Volume: 0.6 mL

Additional Information: Do not aliquot into viral transport media.

 

Acceptable:

Specimen Type: Oropharyngeal (throat) swab, nasal mid-turbinate, or nares/nasal swab

Supplies:

-Culturette (BBL Culture Swab) (T092)

-Swab, Sterile Polyester (T507)

Collection Container/Tube:

Preferred: BBL Culture Swab or COPAN Mid-Turbinate Swab

Acceptable: Dacron-tipped swab with plastic handle

Submission Container/Tube: Swab in sterile container with transport media

Specimen Volume: Entire specimen with a minimum of 1.5 mL (maximum 3 mL) of transport media

Collection Instructions: Swab must be placed into viral transport media (eg, M4-RT, M4, or M5), saline, or PBS. Media should not contain guanidine thiocyanate (GTC).

 

Specimen Type: Bronchial washings, endotracheal aspirate, sputum

Container/Tube: Sterile container

Specimen Volume: 0.6 mL

Additional Information: Do not aliquot into viral transport media.


Useful For

Simultaneous detection of influenza A virus, influenza B virus, and respiratory syncytial virus in upper or lower respiratory tract specimens from individuals with flu-like illnesses

Method Name

Multiplex Reverse Transcriptase, Real-Time Polymerase Chain Reaction (RT-qPCR)

Reporting Name

Influenza A/B and RSV, PCR, Varies

Specimen Type

Varies

Specimen Minimum Volume

Upper respiratory tract swab: See Specimen Required; Lower respiratory specimens: 0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Frozen (preferred) 14 days
  Refrigerated  72 hours

Reject Due To

Bloody specimen Reject
Calcium alginate-tipped swab Reject
Wooden shaft swab Reject
Dry swab Reject
Glass transport media tubes Reject
Transport swab containing gel or charcoal additive Reject
Sample tubes containing guanidine isothiocyanate, guanidine thiocyanate, or guanidine hydrochloride Reject

Reference Values

Undetected

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Specimen Retention Time

4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87631

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HPFLU Influenza A/B and RSV, PCR, Varies 92143-7

 

Result ID Test Result Name Result LOINC Value
HPFLS Influenza A/B and RSV, Source 31208-2
610412 Influenza A virus PCR 92142-9
610413 Influenza B virus PCR 92141-1
610414 Respiratory Syncytial Virus, PCR 92131-2

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send an Microbiology Test Request (T244) with the specimen.